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phospho-PTPN6 (Tyr536)/FITC (bs-5577R-FITC)
  • datasheet
  • 概述
    产品编号
    bs-5577R-FITC
    产品分类
    抗体 / 标记一抗 / FITC
    英文名称
    phospho-PTPN6 (Tyr536)/FITC
    中文名称
    FITC标记的磷酸化蛋白酪氨酸磷酸酶1C抗体
    英文别名
    SHP-1(Phospho-Tyr536); PTPN6(phospho Y536); SHP1(phospho Y536); SHP1; 70 kda SHP 1L protein; 70 kda SHP1L protein; 70Z-SHP; EC 3.1.3.48; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP 1C; HPTP1C; MGC124580; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP 1; Protein tyrosine phosphatase SHP1; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6_HUMAN; PTP 1C; PTP-1C; PTP1C; PTPN 6; PTPN6; SH PTP 1; SH PTP1; SH

     

    交叉反应
    (predicted: Horse, Dog, Human)
    标记
    FITC

     

    抗体来源
    Rabbit
    抗体类型
    Polyclonal
    免疫原
    KLH conjugated Synthesised phosphopeptide derived from human PTPN6 around the phosphorylation site of Tyr536
    蛋白细胞定位
    细胞核
    纯化方法
    affinity purified by Protein A
    亚型
    IgG
    性状
    Liquid
    浓度
    1mg/ml
    储存液
    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件
    Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    PubMed
    PubMed
    研究领域

       

      背景资料
      The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]

       

      GeneID
      5777
      Swiss
      P29350
      Function
      Modulates signaling by tyrosine phosphorylated cellcsurface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
      Subunit
      Monomer. Interacts with MTUS1. Interacts with MILR1 (tyrosine-phosphorylated). Interacts with KIT. Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, FCRL3, FCRL4, CD300LF, CDK2 and CD84. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1.
      Subcellular Location
      Cytoplasm. Nucleus. Note=In neurons, translocates into the nucleus after treatment with angiotensin II. Shuttles between the cytoplasm and nucleus via its association with PDPK1.
      Tissue Specificity
      Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
      Post-translational modifications
      Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation. Phosphorylation at Tyr-564 enhances phosphatase activity.
      Similarity
      Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
      Contains 2 SH2 domains.
      Contains 1 tyrosine-protein phosphatase domain.
      Database links

      Entrez Gene: 5777 Human

      Entrez Gene: 15170 Mouse

      Entrez Gene: 116689 Rat

      Omim: 176883 Human

      SwissProt: P29350 Human

      SwissProt: P29351 Mouse

      SwissProt: P81718 Rat

      Unigene: 63489 Human

      Unigene: 271799 Mouse

      Unigene: 18985 Rat

      Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    • 相关标记抗体
      产品名称 产品编号 规格 价格
      phospho-PTPN6 (Tyr536)/AP bs-5577R-AP 2980.00
      phospho-PTPN6 (Tyr536)/APC bs-5577R-APC 2980.00
      phospho-PTPN6 (Tyr536)/APC-Cy5 bs-5577R-APC-Cy5 2980.00
      phospho-PTPN6 (Tyr536)/APC-Cy5.5 bs-5577R-APC-Cy5.5 2980.00
      phospho-PTPN6 (Tyr536)/APC-Cy7 bs-5577R-APC-Cy7 2980.00
      phospho-PTPN6 (Tyr536)/Bio bs-5577R-Bio 2980.00
      phospho-PTPN6 (Tyr536)/Cy3 bs-5577R-Cy3 2980.00
      phospho-PTPN6 (Tyr536)/Cy5 bs-5577R-Cy5 2980.00
      phospho-PTPN6 (Tyr536)/Cy5.5 bs-5577R-Cy5.5 2980.00
      phospho-PTPN6 (Tyr536)/Cy7 bs-5577R-Cy7 2980.00
      phospho-PTPN6 (Tyr536)/FITC bs-5577R-FITC 2980.00
      phospho-PTPN6 (Tyr536)/HRP bs-5577R-HRP 2980.00
      phospho-PTPN6 (Tyr536)/RBITC bs-5577R-RBITC 2980.00
      phospho-PTPN6 (Tyr536)/PerCP bs-5577R-PerCP 2980.00
      phospho-PTPN6 (Tyr536)/PerCP-Cy5.5 bs-5577R-PerCP-Cy5.5 2980.00
      phospho-PTPN6 (Tyr536)/PerCP-Cy7 bs-5577R-PerCP-Cy7 2980.00
      phospho-PTPN6 (Tyr536)/PE bs-5577R-PE 2980.00
      phospho-PTPN6 (Tyr536)/PE-Cy3 bs-5577R-PE-Cy3 2980.00
      phospho-PTPN6 (Tyr536)/PE-Cy5 bs-5577R-PE-Cy5 2980.00
      phospho-PTPN6 (Tyr536)/PE-Cy5.5 bs-5577R-PE-Cy5.5 2980.00
      phospho-PTPN6 (Tyr536)/PE-Cy7 bs-5577R-PE-Cy7 2980.00
      phospho-PTPN6 (Tyr536)/BF350 bs-5577R-BF350 2980.00
      phospho-PTPN6 (Tyr536)/BF405 bs-5577R-BF405 2980.00
      phospho-PTPN6 (Tyr536)/BF488 bs-5577R-BF488 2980.00
      phospho-PTPN6 (Tyr536)/BF555 bs-5577R-BF555 2980.00
      phospho-PTPN6 (Tyr536)/BF594 bs-5577R-BF594 2980.00
      phospho-PTPN6 (Tyr536)/BF647 bs-5577R-BF647 2980.00
      phospho-PTPN6 (Tyr536)/BF680 bs-5577R-BF680 2980.00
      phospho-PTPN6 (Tyr536)/BF750 bs-5577R-BF750 2980.00
    • 应用
      我们在涵盖以下测试应用中使用 bs-5577R-FITC
      应用笔记包括推荐的起始稀释液; 最佳稀释度/浓度应由最终用户确定。
      应用 评级 推荐稀释比例
      Flow-Cyt 2ug/test.
    • 图片资料
      1. Blank control: HL60.
        Primary Antibody (green line): Rabbit Anti-phospho-PTPN6(Tyr564) antibody (bs-5577R-FITC)
        Dilution: 0.2μg /10^6 cells;
        Isotype Control Antibody (orange line): Rabbit IgG-FITC .
        Protocol
        The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature . The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    提示:发表研究结果有使用 bs-5577R-FITC 时请让我们知道,以便我们可以引用参考文章。作为回馈,资料提供者将获得我们送上的小礼品。
    具体参考文献:bs-5577R-FITC 被引用于 0 文献中。
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